Fig. 2: GCTs formed in DKO females with relatively low incidence in a short time frame. | Cell Death & Differentiation

Fig. 2: GCTs formed in DKO females with relatively low incidence in a short time frame.

From: Lineage tracing of mutant granulosa cells reveals in vivo protective mechanisms that prevent granulosa cell tumorigenesis

Fig. 2

A Representative hematoxylin-stained sections of ovaries from NoCre control mice and DKO mice at two months after completion of Tam treatment, showing numerous cyst-like structures with cavities appearing in the ovaries of DKO mice. C indicates cyst-like structures. Red arrows indicate residual cells. The boxed regions in the left panels are magnified in the right panels. Scale bar: 500 μm. B Immunofluorescence staining of FOXL2 (GC marker, red) and Hoechst (nuclear stain, blue) in ovaries of NoCre control and DKO mice, showing residual cells were FOXL2 positive. The boxed regions in the left panels are magnified in the right panels. C indicates cyst-like structures. The red arrow indicates residual FOXL2-positive cells. Scale bar: 100 μm. C Representative images of genital ducts and ovaries dissected from NoCre control and DKO mice, showing an ovarian tumor that developed in a DKO mouse at three to four months. Red arrows indicate the normal ovary (left) and ovarian tumor (right). D Representative hematoxylin-stained sections of ovaries showing nest structures with clear outlines in a DKO ovarian tumor compared to a control ovary dissected from a NoCre control mouse of similar age. Scale bar: 500 μm. E Immunofluorescence staining of FOXL2 (red) and Hoechst (blue) in ovaries of NoCre control and DKO mice, showing that the tumor cells that developed in DKO females were FOXL2 positive. Scale bar: 100 μm. F PCR results showing that there was no Pten or p27 in the GCT cell genome DNA from DKO mice compared to the NoCre control GC genome DNA. G Immunoblot results showing that PTEN and P27 were not expressed in GCT cells from DKO mice compared to NoCre control mice GCs. H Statistical results showing that the incidence of GCTs in DKO mouse ovaries was 33.3% (19/57) during the nine months of tracing. I Statistical results showing the number of GCT cases detected at the indicated days, illustrating that GCT tumorigenesis was concentrated in a short time frame from three to four months.

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