Fig. 5: iASPP regulates its surrounding immune microenvironment in response to inflammation and oncogenic KRAS. | Cell Death & Differentiation

Fig. 5: iASPP regulates its surrounding immune microenvironment in response to inflammation and oncogenic KRAS.

From: p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation-driven pancreatic cancer

Fig. 5

A Cytokine protein ELISA array was performed on murine pancreatic tissue lysates following six weekly doses of caerulein. B Immune cell profiling of KC (n = 13) and KC;iASPPΔ8/Δ8 (n = 10) pancreatic tissue samples following six weekly doses of caerulein determined by flow cytometry. Mean number of immune cells per pancreas ± SEM is shown by horizontal lines. MDSC-M: CD11b + Ly6C + Ly6Glo, MDSC-P: CD11b + Ly6cloLy6G+, Monocyte: CD11b + Ly6GloLy6Clo, Neutrophil: CD11b + Ly6G + Ly6C+, DC: CD19-CD11c + MHCII + , Macrophage: CD19-CD68 + F4/80+, NK: CD19-CD49b+, B: CD19+, CD4: CD45 + CD3 + CD4+, CD8: CD45 + CD3 + CD8+, Treg: CD45 + CD3 + CD4 + FoxP3+, γδT: CD45 + CD3 + TCRγδ+. ** p = 0.015, * p < 0.01, † p = 0.042. 11x, 21x, 36x, 20x and 32x signifies fold change in KC;iASPPΔ8/Δ8 relative to KC. Statistical analysis was carried out by multiple unpaired t tests.

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