Fig. 6: iASPP suppresses PC growth through both cell intrinsic and extrinsic pathways.
From: p53 inhibitor iASPP is an unexpected suppressor of KRAS and inflammation-driven pancreatic cancer
A Schematic diagram for the experiment; dissection of PC (left), derivation of primary PC cell lines (centre) and tumours at culling following bilateral flank subcutaneous injections (right). B Left column, representative images of primary PC from three KC and three KC;iASPPΔ8/Δ8 mice used to generate cell lines. Second left column, representative phase contrast imaging of corresponding primary pancreatic cancer cell lines. Second right column, representative images of excised syngeneic-injected tumour. Right column, representative images of excised nude-injected tumour. Scale bars 100 µm. C Mean weights ± SEM of syngeneic and nude subcutaneous tumours for KC-1, KC-2, KC-3 and KC;iASPPΔ8/Δ8-1, KC;iASPPΔ8/Δ8-2, KC;iASPPΔ8/Δ8-3 cell lines. **** = p < 0.0001; *** = p < 0.001; ** = p < 0.01; * = p < 0.05; n.s., not significant. P values were calculated using unpaired t-test. D Average weights for KC and KC;iASPPΔ8/Δ8 cell lines tumours in BL6 and nude mice. ** = p < 0.01; * = p < 0.05; n.s., not significant. P values were calculated using unpaired t-test.
