Fig. 2: TMEM11 mediates cardiomyocytes proliferation in adult hearts. | Cell Death & Differentiation

Fig. 2: TMEM11 mediates cardiomyocytes proliferation in adult hearts.

From: TMEM11 regulates cardiomyocyte proliferation and cardiac repair via METTL1-mediated m7G methylation of ATF5 mRNA

Fig. 2

a Western blot image (top) and statistical data (bottom) showing the myocardial level of TMEM11 in wild type (WT) and TMEM11 KO adult mice hearts (n = 8 mice). b–h TMEM11 silencing increases cardiomyocytes proliferation in adult mice hearts. Representative images of gross morphology and haematoxylin-eosin stained transverse sections of hearts from WT and TMEM11 KO mice (Bar = 2 mm) (b). Heart to body weight ratio (c) and echocardiography analysis of fractional shortening (FS) (d) in WT and TMEM11 KO mice (n = 7 mice). e Representative confocal images of Ki67-positive cardiomyocytes (red, Bar = 25 μm) and quantification of Ki67-positive (right) cells (n = 7 mice per group). cTNT (green) mark cardiomyocytes and DAPI (blue) labels nuclei. f, g Representative confocal images and qualification of pH3-positive cardiomyocytes (red, Bar = 25 μm) in WT and TMEM11 KO hearts. h Aurora-B-positive (red) cardiomyocytes was calculated in WT and TMEM11 KO hearts. cTNT marks cardiomyocytes and DAPI labels nuclei (Bar = 25 μm). (n = 6 mice per group). Data are presented as the mean ± s.d. Two-sided Student’s t test (a, c–e, g and h) was performed.

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