Fig. 6: C/EBPα regulated the transcription of RTN3.

a Quantitative analysis of RTN3 mRNA level in NRVCs treated with Pal (n = 6 wells each group); b Representative western blotting images and quantitative analysis of RTN3 protein level (n = 6 wells each group); c Quantitative analysis of RTN3 mRNA level in the hearts of ND- and HFD-fed mice (n = 6 mice each group); d Venn diagram showing transcription factors identified with three databases; e Correlation between the mRNA level of C/EBPα and C/EBPβ and RTN3 based on a public data set (GSE40722); f ChIP analysis for C/EBPα binding to the RTN3 promoter in NRVCs; g q-PCR analysis of DNA obtained from ChIP (n = 3 each group); h Relative luciferase activity of cells infected with different plasmids (n = 3 wells each group); i Relative luciferase activity of cells infected with plasmids expressing truncated RTN3 promotor region and C/EBPα (n = 3 wells each group); j Schematic diagrams of wild type and deleted forms of RTN3 promotor region; k Relative luciferase activity of cells infected with plasmids expressing wild type and deleted forms of RTN3 promotor region and C/EBPα (n = 3 wells each group); l q-PCR analysis of DNA obtained from ChIP with or without palmitate treatment; m Representative western blotting images and quantitative analysis of C/EBPα protein level (n = 6 mice each group); n Representative western blotting images and quantitative analysis of RTN3 protein level (n = 6 wells each group); Representative fluorescence images indicating LDs labeled with Bodipy 493/503 and nucleus labeled with DAPI (o) and quantitative analysis of LDs area per cell (p) (n = 10 images each group), scale bar = 10 μm. Data are expressed as Mean ± SEM. Differences are significant for *P < 0.05, **P < 0.01.