Fig. 4: Parkin translocation induced by Calcineurin is Miro1-dependent. | Cell Death & Differentiation

Fig. 4: Parkin translocation induced by Calcineurin is Miro1-dependent.

From: Activation of Ca2+ phosphatase Calcineurin regulates Parkin translocation to mitochondria and mitophagy in flies

Fig. 4

A Representative confocal images of Ctrl siRNA MEF cells transfected with mCherry-Parkin, mito-YFP and with empty vector (EV) or constitutively active CaN (ΔCnA) for 2 days before being treated with DMSO or 10 μM CCCP for 3 h. B Quantification of A using Squassh. The graph bars show mean ± SEM of Squassh colocalization coefficient for at least ≥ 10 images per biological replicate. 0 = no colocalization, 1 = perfect colocalization. Two-way ANOVA followed by Holm-Sidak multiple comparison test (n = 3; p < 0.01). C Representative confocal images of Miro1 siRNA MEF cells transfected with mCherry-Parkin, mito-YFP and with empty vector (EV) or constitutively active CaN (ΔCnA) for 2 days before being treated with DMSO or 10 μM CCCP for 3 h. D Quantification of C using Squassh. The graph bars show mean ± SEM of Squassh colocalization coefficient for at least ≥ 10 images per biological replicate. 0=no colocalization, 1 = perfect colocalization. Two-way ANOVA followed by Holm-Sidak multiple comparison test (n = 3; p < 0.01).

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