Fig. 6: Parkin translocation is induced by Calcineurin independently of Drp1 mitochondrial recruitment and activity. | Cell Death & Differentiation

Fig. 6: Parkin translocation is induced by Calcineurin independently of Drp1 mitochondrial recruitment and activity.

From: Activation of Ca2+ phosphatase Calcineurin regulates Parkin translocation to mitochondria and mitophagy in flies

Fig. 6

A Representative confocal images of wild type MEF cells transfected with mCherry-Parkin and YFP-Drp1 or YFP-Drp1 S637A plus dominant negative CaN (ΔCnAH151Q) for 2 days before being treated with DMSO or 10 μM CCCP for 3 h. B Quantification of A. Graph bar shows mean ± SEM of percentage of cells with mCherry-Parkin puncta for ≥ 300 cells per biological replicate. Two-way ANOVA followed by Tukey’s multiple comparison test (n = 3). C Representative confocal images of wild type MEF cells transfected with mCherry-Parkin, mito-YFP and constitutive active CaN (ΔCnA) plus Drp1 or Drp1 K38A for 2 days before being treated with DMSO or 10 μM CCCP for 3 h. D Quantification of C. Graph bar shows mean ± SEM of percentage of cells with mCherry-Parkin on mitochondria for ≥ 300 cells per biological replicate. Two-way ANOVA followed by Tukey’s multiple comparison test (n = 3).

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