Fig. 8: Calcineurin is required for CCCP-induced mitophagy. | Cell Death & Differentiation

Fig. 8: Calcineurin is required for CCCP-induced mitophagy.

From: Activation of Ca2+ phosphatase Calcineurin regulates Parkin translocation to mitochondria and mitophagy in flies

Fig. 8

A Representative image of mt-Keima expressing cells acquired on confocal microscope Operetta High-Content Imaging system. MEFs were transfected with constitutive active CaN (ΔCnA) or empty vector (EV). B mt-Keima analysis in MEFs expressing constitutive active CaN (ΔCnA) and relative control (EV). Two-way ANOVA followed by Tukey’s multiple comparison test (n = 6; p < 0.01). C Representative electron microscope images showing the presence of autophagosomes with mitochondrial-like structures inside in MEFs expressing constitutive active CaN (ΔCnA) and control (EV). 24 h CCCP-treated cells were used as positive control. Mitochondrial-like structures were identified as described in Chakraborty et al. [130]. D Bar graph represents mean ± SEM of the number of autophagosomes and autolysosomes per cell. At least 60 cells per biological replicate were analyzed from each group. Student’s t-test (n = 3-4; p < 0.01).

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