Fig. 2: ABLIM1 silence inhibits CRC growth and metastasis in vitro and in vivo. | Cell Death & Differentiation

Fig. 2: ABLIM1 silence inhibits CRC growth and metastasis in vitro and in vivo.

From: ABLIM1, a novel ubiquitin E3 ligase, promotes growth and metastasis of colorectal cancer through targeting IĸBα ubiquitination and activating NF-ĸB signaling

Fig. 2

A Western blot validation of the knock-down efficiency of lenti-virus shABLIM1#1 and shABLIM1#2 in HCT116 and RKO cells. B Cell proliferation indexes of stable shABLIM1 HCT116, RKO, and control shVec cells detected by Real-time cell analysis (RTCA) xCELLigence® system. For HCT116, n = 3 or 4 for each group. For RKO, n = 5 for each group. C HCT116 and RKO cell colonies formed in 6-well plates after 10-day culture were visualized by crystal violet staining. Cell migration assays using the RTCA system and Transwell chambers in HCT116 cells D and RKO cells E. For HCT116, n = 3 or 4 for each group. For RKO, n = 5 or 6 for each group. Cell invasion assays using the RTCA system and Transwell chambers in HCT116 cells F and RKO cells G. For HCT116, n = 3 or 4 for each group. For RKO, n = 7 for each group. For n > 3, paired student’s t-test; For n = 3, Friedman test in B–G. H Effect of ABLIM1 knockdown on CRC xenograft growth in nude mice. Stable shVec or shABLIM1 HCT116 cells were subcutaneously injected into nude mice (n = 6) and the tumor xenografts were photographed after sacrificing the mice on day 27 after inoculation. Unpaired student’s t-test. I Effect of ABLIM1 knockdown on CRC liver metastasis in nude mice. Stable shVec or shABLIM1 SW620 cells were injected into the hepatic portal veins of nude mice (n = 5) and the liver metastasis nodes were photographed after sacrificing the mice on day 25. Blue triangles indicate the tumor nodes in livers. Mann Whitney test. *P < 0.05; **P < 0.01; ***P < 0.001.

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