Fig. 3: ACSS2 is recruited to acetylated PPARγ in a ligand-dependent manner.
From: ACSS2 controls PPARγ activity homeostasis to potentiate adipose-tissue plasticity
A ACSS2-PPARγ protein docking using Z-Dock server. Model represents the best among all the docking poses. Protein colored green is ACSS2 and protein colored purple is PPARγ; Protein colored yellow is RXRα; The white colored helix is PPRE elements DR1; Rosiglitazone (Left) and GW9662 (Right) are blue balls inside PPARγ; The blue balls around DNA are Zinc ion; The gray balls are 9-cis-retinoic acid for RxRα ligand. The brown sticks are NCoA2 Peptide around PPARγ and RXRα. B Representative immunoblot of exogenous IP of FLAG-tagged ACSS2 and HA-tagged PPARγ in response to rosiglitazone (0–10 μM) and GW9662 (0–10 μM) for 16 h in HEK293T cells (n = 3 biological replicates). C Representative BiFC fluorescent images of HEK293T cells transfected with 2 μg plasmid encoding ACSS2 or PPARγ fused to the fluorescent protein fragments indicated in each panel in response to 10 μM rosiglitazone and GW9662 alone or together for 16 h (n = 3 biological replicates). D Representative immunoblot of exogenous IP of FLAG-tagged ACSS2 or its N-terminal mutants with HA-tagged PPARγ in HEK293T cells (n = 3 biological replicates). E Representative BiFC fluorescent images of FLAG-tagged ACSS2 or its N-terminal mutants with HA-tagged PPARγ in HEK293T cells (n = 3 biological replicates). F Representative immunoblot of exogenous IP of FLAG-tagged ACSS2 or its C-terminal mutants with HA-tagged PPARγ in HEK293T cells (n = 3 biological replicates). G Representative BiFC fluorescent images of FLAG-tagged ACSS2 or its C-terminal mutants with HA-tagged PPARγ in HEK293T cells (n = 3 biological replicates). H Representative immunoblot of exogenous IP of FLAG-tagged ACSS2, HA-tagged PPARγ and HA-P300 in HEK293T cells (n = 3 biological replicates). I Representative immunoblot of exogenous IP of FLAG-tagged ACSS2, HA-tagged PPARγ and MYC-tagged P300 in HEK293T cells expressing PPARγ in response to increasing P300 and ACSS2 individually (n = 3 biological replicates). J Representative immunoblot of exogenous IP of HA-tagged PPARγ, P300, ACSS2 and acetylated proteins following transfection with PPARγ and P300 in HEK293T cells (n = 3 biological replicates).
