Fig. 2: HSPC multilineage potential and lineage differentiation is regulated by BRD9.

A, B Methylcellulose colony formation capacity of HSPCs infected with control or BRD9 shRNA (n = 4) and in presence of DMSO or increased concentrations of dBRD9A (n = 6) (plots show individual data points with mean and SD; and p values from unpaired t-tests compared to control from one biological replicate). HSPC proliferation and differentiation in megakaryocytic (C), erythroid (D), and myeloid media (E) (all technical replicates with their mean and SEM from one of the n = 5 independent experiments are plotted and p values from unpaired t-test between shNT and BRD9 shRNA conditions are shown). F FACS sort gating strategy for GFP+ cells in shNT and shBRD9 infected cells using CD34 and CD45RA along differentiation trajectory, where Q1 depicts the least and Q3 the most differentiated state. Cell proliferation and differentiation in G megakaryocytic lineage promoting media showing cell count and CD41 expression, H erythroid media with cell counts and CD71 expression and I myeloid media with relative cell proliferation and CD15 surface expression from sorted fractions (plots show individual data points, mean and SEM and p values from unpaired t-tests compared to control as *<0.05, **<0.005, ***<0.0005 and ****<0.00005, also see methods section) .