Fig. 1: OGT expression is critical for colorectal tumor growth and metabolism.
From: The OGT–c-Myc–PDK2 axis rewires the TCA cycle and promotes colorectal tumor growth
A Quantification analysis of OGT expression levels from human colon tumor (T) and the matching non-tumor (N) tissue samples (n = 30). B Immunohistochemistry staining of human CRC samples with OGT antibody (Scale bar, 100 μm). The relative OGT expression was quantified based on intensity and area of the staining. C Immunoblotting analysis of OGT expression using colon cancer cell lines (HCT116, HT-29, RKO, SW620 and LoVo) and a normal colon cell line (NCM460). D Cell proliferation of NCM460, HT-29, HCT116 cells upon OGT knockdown with small hairpin RNAs (shRNA1 and 2). n = 5; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. E HT-29 cells expressing scramble or shOGT were subcutaneously injected into 6-week-old male nude mice (n = 5 per group). Images of dissected tumors from mice. F Growth curves of tumors measured at indicated time points. n = 5; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. G Tumor mass measured at the experimental endpoint. n = 5; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. H Ki67 staining of tumors generated from HT-29 cells infected with scramble or shOGT (Scale bar, 100 μm). Quantification was shown. n = 5; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. I Analysis of extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in HT-29 cells expressing scramble or shOGT. n = 3; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. Analysis of ROS (J), NADPH (K), GSH/GSSG (L) in HT-29 cells expressing scramble or shOGT. n = 3; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. Relative abundance of metabolites derived from glycolysis (M) and the TCA cycle (N) in HT-29 cells expressing scramble or shOGT. n = 3; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests.
