Fig. 2: OGT depletion upregulates the TCA cycle by transcriptional repression of PDK2.
From: The OGT–c-Myc–PDK2 axis rewires the TCA cycle and promotes colorectal tumor growth
A GSEA analysis of OGT-regulated gene signature versus glucose metabolic process. B Heatmap showing the top 10 downregulated genes of glucose metabolism from the RNA-seq analysis. Relative abundance of metabolites derived from glycolysis (C) and TCA cycle (D) HT-29 cells expressing scramble or shPDK2. n = 3; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. Comparison of ROS (E), NADPH (F), GSH/GSSG (G) in HT-29 cells expressing scramble, shOGT or shOGT reconstituted with PDK2 expression. n = 3; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. Cell proliferation (H) and clone formation (I) of HT-29 cells expressing scramble, shOGT or shOGT with reconstituted PDK2 expression. n = 5; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. Quantitative PCR analysis (J) and immunoblotting analysis (K) of PDK2 expression in HT-29 cells expressing scramble or shOGT. n = 3; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. Quantitative PCR analysis (L) and immunoblotting analysis (M) of PDK2 expression in HT-29 cells expressing control vector or LV-OGT. n = 3; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests.
