Fig. 6: c-Myc-mediated PDK2 expression is vital for cell proliferation and tumor growth.

Cell proliferation (A) and clone formation (B) of HT-29 cells stably expressing scramble shRNA, c-Myc-targeting shRNA, or reconstituted with WT, S415A c-Myc or S415A c-Myc with PDK2 overexpression. C Xenograft analysis of HT-29 cells stably expressing scramble, c-Myc-targeting shRNA, or reconstituted with WT, S415A c-Myc or S415A c-Myc with PDK2 overexpression. Images of dissected tumors from mice. Analysis of tumor growth (D), tumor weights (E) and Ki67 staining (F) of tumors generated from above HT-29 cells. Scale bars: 100 μm. Quantification was shown. n = 5; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. G Representative ¹⁸F-FDG micro-PET/CT images of mice. Red circles indicate glucose uptake in tumors. n = 5 mice per group. Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. H The statistical analysis of c-Myc glycosylation in paired tumor and matched adjacent tissues. n = 30; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. I Analysis of the correlation of c-Myc O-GlcNAcylation and c-Myc expression levels by western blotting. n = 30; Data are presented as means ± SD. P values were determined by unpaired two-tailed Student’s t tests. J Analysis of the correlation of OGT expression and c-Myc O-GlcNAcylation by western blotting. K–M Analysis of the correlation of OGT, c-Myc and PDK2 protein levels in clinical CRC specimens. Analysis of the correlation of OGT / PDK2 (K), OGT / c-Myc (L), and c-Myc / PDK2 (M) from CRC samples. Representative IHC staining of OGT and c-Myc in 30-paired cases of CRC samples (N). Scale bars: 200 μm. Right panels are ×4 magnification of the dashed areas on the left. P values were determined by unpaired two-tailed Student’s t tests.