Fig. 4: Global RNA expression pattern and pro-inflammatory signaling pathways upon CAD activation in HaCaT-ICAD cells.
From: The Caspase-Activated DNase drives inflammation and contributes to defense against viral infection
HaCaT ICAD-mAID-GFP cells and STING-deficient HaCaT ICAD-mAID-GFP cells were stimulated with auxin or with solvent for 6 h or 14 h and subjected to RNA-sequencing. A Volcano plot of differentially expressed genes in HaCaT ICAD-mAID-GFP cells showing significantly upregulated and downregulated genes at 6 h (6 h auxin vs. solvent) and14 h (14 h auxin vs. solvent) of stimulation (top two panels). Bottom panels, the same analysis for HaCaT ICAD-mAID-GFP cells lacking STING (14 h). B Significantly upregulated genes that are unique to HaCaT ICAD-mAID-GFP cells or STING-deficient HaCaT ICAD-mAID-GFP cells or shared between both genotypes. Top, gene expression after 6 h, bottom after 14 h of stimulation. C Heatmap diagram of log2 fold change values of gene expression at different time points with respect to their solvent controls after 6 and 14 h of CAD activation. Shown are significantly differentially regulated innate immunity genes (InnateDB database) following protein-protein-interaction network analysis. DMSO, comparison of DMSO 14 vs. 6 h. D Pathway enrichment analysis of genes represented in protein-protein-interaction networks (Fig. S5) following CAD activation. A–D, Genes with adjusted p-values < 0.05 and log2 fold change value <-0.6 or >0.6 are considered as significantly differentially expressed. P value < 0.05 was adjusted to consider only statistically significant pathways.
