Fig. 5: ATAT1, as the lactylation writer of NAT10 promotes viral lytic transcripts translation and virus reactivation. | Cell Death & Differentiation

Fig. 5: ATAT1, as the lactylation writer of NAT10 promotes viral lytic transcripts translation and virus reactivation.

From: Lactylation of NAT10 promotes N4‐acetylcytidine modification on tRNASer-CGA-1-1 to boost oncogenic DNA virus KSHV reactivation

Fig. 5

A The expression levels of vIRF1 and K-bZIP in iSLK-KSHV cells transduced with lentiviral sgATAT1 (sgATAT1 #2, sgATAT1 #3) or control lentivirus (Lenti-V2) for 72 h were examined by Western blot with the corresponding antibodies. B The RT-qPCR analysis for the ribosome-nascent chain-complex-bound mRNA (RNC-qPCR) of representative viral genes of KSHV (K5, K8, vIRF1, vIL-6, vBCL-2, and ORF65) in cells treated as in (A). *P < 0.05, **P < 0.01 and ***P < 0.001 by Student’s t-test. C By the assessment of ORF26, real-time DNA-PCR for cells treated as in (A) was performed to examine viral copy number after doxycycline stimulation for 72 h. ***, P < 0.001 by Student’s t-test. D The RT-qPCR analysis for the ribosome-nascent chain-complex-bound mRNA (RNC-qPCR) of representative viral genes of KSHV (RTA, K5, K8, ORF45, ORF57, vIRF1, vIL-6, vBCL-2, ORF65, and K8.1) in iSLK-KSHV cells transduced with lentiviral ATAT1 (ATAT1) or its control (pCDH) for 48 h. ***, P < 0.001 by Student’s t-test. E By the assessment of ORF26, real-time DNA-PCR for cells treated as in (D) was performed to detect viral copy number after doxycycline stimulation for 72 h. **P < 0.01 by Student’s t-test.

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