Table 1 Characteristics of three clusters of MASLD and controls derived from unsupervised clustering based on hepatic markers of ferroptosis.
Characteristics | Cluster 1 | Cluster 2 | Cluster 3 | p value | Adjusted p value |
|---|---|---|---|---|---|
(n = 7) | (n = 10) | (n = 10) | |||
Liver histology | |||||
Controls, n (%) | 1 (20) | 0 (0) | 4 (80) | 0.1459 | |
MASL, n (%) | 3 (37.5) | 3 (37.5) | 2 (25) | ||
MASH F0-1, n (%) | 3 (42.8) | 3 (42.8) | 1 (14.4) | ||
MASH F2-3, n (%) | 0 (0) | 4 (57.2) | 3 (42.8) | ||
Positive ferrous iron stain, n (%) | 1 (14.3) | 7 (70) | 1 (10) | 0.014 | |
Age, year | 49 (39.8–58.5) | 53 (48.0–58.0) | 46 (36–56.5) | 0.516 | 0.695 |
Gender, male, n (%) | 3 (42.9) | 8 (80) | 5 (50) | 0.298 | 0.596 |
BMI, kg/m2 | 29.0 (27.0–33.0) | 29.6 (29.4–31.6) | 38.0 (35.3–44.5) | 0.546 | 0.695 |
Type 2 diabetes mellitus, % | 4 (57.1) | 5 (50) | 8 (80) | 0.471 | 0.695 |
HOMA-IR | 4.6 (3.2–7.3) | 5.9 (4.1–9.8) | 3.7 (2.9–5.7) | 0.39 | 0.683 |
Total cholesterol, mg/dL | 202 (179.3–219.8) | 141 (137.0–207.0) | 189 (171.5–208.0) | 0.679 | 0.76 |
LDL cholesterol, mg/dL | 105 (92.9–130.0) | 99 (90.0–169.0) | 114 (101.5–151.0) | 0.706 | 0.76 |
HDL cholesterol, mg/dL | 50 (43.5–60.8) | 33 (29.5–38.0) | 50 (44.0–63.5) | 0.022* | 0.308 |
Ferritin, µg/L | 155 (103.5–205.3) | 236 (122.0–262.08) | 113 (66.0–407.5) | 0.76 | 0.76 |
Perls stain, positive, n (%) | 2 (28.6) | 0 (0) | 1 (10) | 0.238 | 0.555 |
AST, U/L | 21 (17.3–29.5) | 37 (34.0–49.0) | 42 (20.0–49.5) | 0.115 | 0.403 |
ALT, U/L | 35 (31.0–47.5) | 67 (57.0–106.0) | 48 (32.0–98.5) | 0.099 | 0.403 |
Cytokeratin-18 M65, U/L | 246.4 (187.1–819.8) | 371.8 (257.9–469.6) | 178.3 (115.3–364.2) | 0.162 | 0.454 |
Cytokeratin-18 M30, U/L | 286.3 (161.4–379.9) | 341.1 (218.0–546.9) | 184.8 (136.3–255.8) | 0.1 | 0.403 |
