Fig. 4: LMO7 promotes the differentiation and chemotaxis of Tregs through TGF-β and facilitates the chemotaxis of Tregs through CCL5. | Cell Death & Differentiation

Fig. 4: LMO7 promotes the differentiation and chemotaxis of Tregs through TGF-β and facilitates the chemotaxis of Tregs through CCL5.

From: LIM domain only 7: a novel driver of immune evasion through regulatory T cell differentiation and chemotaxis in pancreatic ductal adenocarcinoma

Fig. 4

a Differential gene convolution heat map. b Scanning image of Human Th1/Th2/Th17 Array in Vector and LMO7 Groups. c Relative concentration changes of TGF-β protein in cells and their controls. d Transcription levels of TGF-β/CCL5 after LMO7 knockdown. e Extracellular protein levels of TGF-β/CCL5 after LMO7 knockdown. f Transcription levels of TGF-β/CCL5 after LMO7 overexpression. g Extracellular protein levels of TGF-β/CCL5 after LMO7 overexpression. h Flow cytometric analysis of CD4+ FOXP3+ Treg in the differentiation models of CFPAC-1 treated with SiControl + IgG, SiControl + TGF-βAb, SiControl + CCL5Ab, SiLMO7 + Saline, SiLMO7 + rTGF-β, and SiLMO7 + rCCL5. i Flow cytometric analysis of CD4+ FOXP3+ Treg in the differentiation model of Mia Paca-2 treated with Vector + Saline, Vector + rTGF-β, Vector + rCCL5, LMO7 + IgG, LMO7 + rTGF-βAb, and LMO7 + CCL5Ab. j Quantitative analysis of the chemotaxis model of CFPAC-1 treated with SiControl + IgG, SiControl + TGF-βAb, SiControl + CCL5Ab, SiLMO7 + Saline, SiLMO7 + rTGF-β, and SiLMO7 + rCCL5. k Quantitative analysis of the chemotaxis model of Mia Paca-2 treated with Vector + Saline, Vector + rTGF-β, Vector + rCCL5, LMO7 + IgG, LMO7 + rTGF-βAb, and LMO7 + CCL5Ab. Data are presented as mean ± SEM (n = 3 independent biological replicates). Statistical significance was determined using two-tailed unpaired t-test. p < 0.05; **p < 0.01; ***p < 0.001.

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