Fig. 7: LMO7 promotes ubiquitination degradation of Foxp1. | Cell Death & Differentiation

Fig. 7: LMO7 promotes ubiquitination degradation of Foxp1.

From: LIM domain only 7: a novel driver of immune evasion through regulatory T cell differentiation and chemotaxis in pancreatic ductal adenocarcinoma

Fig. 7

a Protein expression of Foxp1 in Mia Paca-2 cells with LMO7 overexpression and control group after cycloheximide treatment. b Protein expression of Foxp1 in CFPAC-1 cells with LMO7 knockdown and control group after cycloheximide treatment. c Protein level changes of Foxp1 after MG132 treatment in SiControl and SiLMO7 group. d Foxp1 was knocked down, and ubiquitin-conjugated Foxp1 was identified via immunoblotting using an anti-HA antibody in LMO7-overexpressed Mia Paca-2 cells and control. e Foxp1 was knocked down, and ubiquitin-conjugated Foxp1 was identified through immunoblotting using an anti-HA antibody in CFPAC-1 cells with LMO7 knockdown and control. Mia Paca-2 cells were transfected with a HIS-LMO7_FL (f) and HIS-LMO7_ΔC (g) plasmid. After 24 h, cell lysates were collected for immunoprecipitation and subsequent western blotting analysis. h Foxp1 protein expression was determined in HIS-LMO7_FL or HIS-LMO7_ΔC Mia Paca-2 cells. i Confocal microscopy showing colocalization of LMO7 (green) with Foxp1 (red). Scale bars, 25 µm. j UMAP plots displaying co-expression analysis of LMO7 and TGFB1. k UMAP plots displaying co-expression analysis of LMO7 and CLL5. l Dot plot of receptor–ligand interactions among ductal cells and immune cells in PDAC with high LMO7 expression using CellphoneDB. m Dot plot of receptor–ligand interactions among ductal cell 2 and immune cells in PDAC using CellphoneDB. n Dot plot of receptor–ligand interactions among ductal cell 2 and immune cells in PDAC with high LMO7 expression using CellphoneDB. n = 3 independent biological replicates.

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