Fig. 6: PRR33 interacts with DESMIN.
From: Adapting cytoskeleton-mitochondria patterning with myocyte differentiation by promyogenic PRR33
A Schematic map of Turbo-ID-based proximal labeling and proteomics profiling. B Gene Ontology-biological process (GO-BP) enrichment analysis. P value < 0.05. C Gene Ontology-cell component (GO-CC) enrichment analysis. P value < 0.05. D Plot of proteins labeled by PRR33-Turbo-ID (iBAQ-biotin vs NC > 3000, Score>250). E BiFC-Venus fluorescence image of ANXA2, ZYX, DESMIN with PRR33. Bar: 400 μm. F Turbo-PRR33 (N) or PRR33-turbo (C) was introduced into C2C12 cells. The biotin-labeled proximal proteins were recovered by Streptavidin-beads and were subjected to Western Blot analysis with indicated antibodies. G Co-immunoprecipitation (Co-IP) experiments to verify the interaction between DESMIN and PRR33. IB FLAG represented the expression of PRR33 in both groups; IP DESMIN showed the interaction of IgG or PRR33 (Over). H Representative immunostaining image of wild type mouse TA muscle section. DESMIN (green), PRR33 (red). N = 3. Bar: 20 μm.
