Fig. 7: PRR33 ablation alters intracellular pattern of DESMIN and mitochondrial morphology.
From: Adapting cytoskeleton-mitochondria patterning with myocyte differentiation by promyogenic PRR33
A Immunostaining images of Prr33fl/fl or Prr33KO TA muscle section at 7 days after BaCl2 injection. DESMIN (green), DAPI (blue), WGA (white). White arrows: accumulated DESMIN. N = 3. Bar: 40 μm. B Percentiles of myofibers showing DESMIN accumulation in the field in TA muscles at 7 days after BaCl2 injection. N = 3. Data was presented as mean ± SEM. (Student’s t-test). C Representative images of immunofluorescence staining for TOMM20 in Prr33fl/fl and Prr33KO TA myofibers. N = 3. Bar: 40 μm. D Distribution of myofibers with normal and abnormal intracellular pattern of mitochondria in Prr33fl/fl and Prr33KO TA muscle samples. N = 3. E Representative images of immunofluorescence staining for TOMM20 (green), DESMIN (red) and DAPI (blue) in C2C12 cells at differentiation day 3. Asterisk (*): accumulated DESMIN; White arrows: disorganized mitochondria. Bar: 30 μm. F Percentiles of myotubes with abnormal accumulated DESMIN and disorganized mitochondria in Ctrl-gRNA and Prr33-gRNA-treated samples.
