Fig. 5: mNAT10 promotes adipogenesis and mKLF9 mRNA ac4C modification.
From: NAT10-mediated N4-acetylcytidine modification in KLF9 mRNA promotes adipogenesis
A mKLF9 motif in acRIP-seq was visualized using IGV software. B The hNAT10 and mNAT10 ac4C motifs, predicted with PACES software. C hNAT10 and mNAT10 ac4C motifs in mRNA 5’-UTR. D acRIP-PCR verified that mNAT10 was able to ac4C modify mKLF9-1 (containing 2 motifs) (n = 4) and (E) mKLF9-2 (n = 4). F acRIP-PCR verified that hNAT10 was able to ac4C modify hKLF9 (n = 4). G The mRNA expression of hKLF9 was upregulated in SATs and VATs of obese (n = 6) compared to normal body weight (n = 6) patients. H, I The protein expression of mKLF9 was upregulated in iWATs and eWATs of high-fat diet group (n = 4) than chew diet group (n = 4). J, K mKLF9 was gradually upregulated during 3T3-L1 adipogenesis (n = 5). L mNAT10 positively regulated mKLF9 mRNA expression (n = 5). M–R mNAT10 positively regulated the protein expression of mKLF9, mPPARG, mCEBPA and mCEBPB in 3T3-L1 (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
