Fig. 7: Remodelin inhibits adipogenesis through mNAT10-mKLF9-mCEBPA/B-mPPARG pathway.
From: NAT10-mediated N4-acetylcytidine modification in KLF9 mRNA promotes adipogenesis
A Remodelin impeded adipogenesis (Oil red O staining) in a dose-depend manner (0–40 μM). B Remodelin impeded cellular FFA contents and TG contents (C) in a dose-depend manner (n = 5), but Remodelin did not affect cell viability (CCK-8) (n = 5) (D). E–M Remodelin significantly inhibited mRNA (n = 5) and protein (n = 3) expression of mPPARG, mCEPBA and mFABP4 (n = 5). N Ac4C dot blot showed that 20 μM Remodelin decreased 3T3-L1 cell’s mRNA ac4C modification. Remodelin inhibited mKLF9 (motif 1 and 2) (O) and (motif 3) mRNA ac4C modification (P), which was detected by using acRIP-PCR (n = 4). Q–S Overexpression of mKLF9 partially increased adipogenesis (Oil red O staining), cellular FFA contents and TC contents after 3T3-L1 cells were treated with 20 μM Remodelin (n = 5). T–Z Overexpression of mKLF9 partially increased the mRNA (n = 5) and protein (n = 3) of mPPARG, mCEBPA and mFABP4 after 3T3-L1 cells were treated with 20 μM Remodelin. *P < 0.05, **P < 0.01, ***P < 0.001.
