Fig. 8: AAV-shRNA-mNAT10 inhibits adipose tissue expansion in vivo.
From: NAT10-mediated N4-acetylcytidine modification in KLF9 mRNA promotes adipogenesis
A The protocol of AAV-shRNA-mNAT10 injection in mice. B Food intake in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups (n = 5). C Body weight in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups (n = 5). D The mice and eWATs and iWATs photos in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups. E The weight of iWATs and eWATs (F) decreased in the AAV-shRNA-mNAT10 group (n = 5). G H&E staining of iWATs in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups. H The adipocytes size of iWATs in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups (n = 40). I acRIP-PCR detected mKLF9 (motif 1 and 2) mRNA ac4C level in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups (n = 4). J acRIP-PCR detected motif 3 mRNA ac4C level in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups (n = 4). K–O The mNAT10, mKLF9, mPPARG, mCEBPA and mFABP4 mRNA expression in iWATs in AAV-shRNA-mNAT10 and AAV-shRNA-mNC groups (n = 5). P, Q The protein expression of mNAT10-mKLF9-mCEBPA/B-mPPARG pathway in iWATs (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.
