Fig. 1: Increased A8 and A9 expression in epidermal and myeloid cells in human and murine skin inflammation.
From: Chronic skin and systemic inflammation modulated by S100A8 and S100A9 complexes
a A8 and A9 expression overlaid on UMAP of scRNA-seq analysis of 46,917 human skin cells. b Mouse A8 and A9 expression overlaid on UMAP of scRNA-seq analysis in skin from BALB/c mice, mock- or topically-treated with SA for 48 h or MC903-treated for 14 days followed by topical SA. c Representative images of skin sections from 6-months-old control and JunB∆ep mice. A8 or A9 (red) immunofluorescence (IF) co-stained with Keratin 5 (K5) (green). Nuclei are stained with DAPI (blue) (top and middle panel). White arrows indicate A8- (top) or A9-positive cells (middle)]. Immunohistochemistry (IHC) for CP (bottom panel). A white (top panels) or black line (bottom panel) indicates the limits of epidermis. Scale bars, 200μm. d A8 dimers, A9 dimers and CP in skin lysates of 2 months-old JunB∆ep mice and control littermates (ELISA). Dot plots represent mean ± SEM. *p ≤ 0.05. Unpaired 2-tailed Student’s t-test with Welch’s correction was applied.
