Fig. 5: Digit swelling and local bone destruction with SA colonization in JunB∆epS100a9−/− mice.
From: Chronic skin and systemic inflammation modulated by S100A8 and S100A9 complexes
a Representative images of hind paws of the indicated mice (top panel) and μCT images of the digits (middle and bottom panels). The black arrow indicates bone destruction in the distal phalanges of JunB∆epS100a9−/− mice. μCT images are representative of at least 3 mice of each group. b SA CFUs in the skin of the digits of all 4 mutant mice. c Representative IF images of SA (red) and K5 (green) in the digits of JunB∆ep and JunB∆epS100a9−/− mice. Nuclei are stained with DAPI (blue). Scale bar, 200μm. The white arrow points to SA penetration. d Percentage of CD45+ neutrophils (CD11b+, Ly6C+, Ly6G+) in the skin surrounding the digits of all mutant mice, analyzed by flow cytometry. e Protein levels of MPO, NE, A8, A9 and CP in skin lysates in the digits of control (n = 4), S100a9−/− (n = 3 < 4), JunB∆ep (n = 9) and JunB∆epS100a9−/− (n = 6) mice normalized ([C]/[Cmax]) by row. The maximum absolute value ([Cmax]) in ng/ml is MPO: 40.33, NE: 25.25, A8: 9.72, A9: 3.44, CP:10.25. f Representative IF images of A8 (red) and K5 (green) in the skin surrounding the digits of JunB∆ep and JunB∆epS100a9−/− mice. Nuclei are stained with DAPI (blue). The white arrow points to A8-positive epidermal cells. A white line indicates the limits of epidermis. Scale bar, 200μm. g Representative IF images of A8-positive cells (green) and Ly6G-positive neutrophils (red) in JunB∆ep and JunB∆epS100a9−/− digits. Nuclei are stained with DAPI (blue). The white arrows point to A8- and Ly6G-stained cells. Scale bar, 200μm. Dot plots represent mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Heat map represents [C]/[Cmax] means, scaled by row, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to control mice, •p ≤ 0.05 ••p ≤ 0.01, •••p ≤ 0.001 compared to S100a9−/− mice and #p ≤ 0.05, ##p ≤ 0.01, ###p ≤ 0.001 compared to JunB∆ep mice. One-way ANOVA with Fishers’ LSD test was used for statistical grouped analysis and unpaired 2-tailed Student’s t-test with Welch’s correction was applied to compare statistical difference between 2 groups (gray stars).
