Fig. 2: In vitro and in vivo synthetic lethality in ARID1A-KO HCT116 cells by c-MET kinase inhibitor. | Cell Death & Differentiation

Fig. 2: In vitro and in vivo synthetic lethality in ARID1A-KO HCT116 cells by c-MET kinase inhibitor.

From: ARID1A loss enhances sensitivity to c-MET inhibition by dual targeting of GPX4 and iron homeostasis, inducing ferroptosis

Fig. 2

A Silencing of MET expression in HCT116 by siRNA (siMET). GAPDH was used as a control. B Synthetic lethality validation with siMET. ARID1A-WT and ARID1A-KO clone was transfected with 100 μM siMET for 72 h and the cell images were taken. Scale bars, 100 μm. C The cell density was determined with Image J software. ANOVA P value of <0.01. D Schematic illustration of mouse tumor xenograft experiments with HCT116 ARID1A-isogenic cell pair. E Tumor growth curve in nude mice bearing HCT116-WT or HCT116 ARID1A-KO xenografts after intravenous injection of vehicle, 15 or 30 mg kg−1 (mpk) PHA. Error bars represent a P value of <0.01 between vehicle and PHA treatment groups (n = 3). Student’s t-test. F Wet weight measurement of the tumors isolated from mice bearing HCT116-WT or HCT116 ARID1A-KO xenografts at 24 days after injection of vehicle, 15 or 30 mpk PHA. Error bars represent s.d.

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