Fig. 5: Synthetic lethality and ferroptosis in ARID1A-deficient RKO cells by PHA treatment. | Cell Death & Differentiation

Fig. 5: Synthetic lethality and ferroptosis in ARID1A-deficient RKO cells by PHA treatment.

From: ARID1A loss enhances sensitivity to c-MET inhibition by dual targeting of GPX4 and iron homeostasis, inducing ferroptosis

Fig. 5

A Expression level of ARID1A in RKO and two selected ARID1A stable clones were detected with immunoblot analysis. B Dose-response curves of parental RKO and ARID1A-overexpressing (ARID1AOE) RKO clones treated with PHA. HCT116 ARID1A-WT cells were used as a positive control. Error bars represent s.d. (n = 5) from three independent experiments. C Synthetic lethality with siMET transfection in RKO isogenic cells. RKO and ARID1AOE clones were transfected with 100 μM siMET for 72 h. Scale bars, 100 μm. D The cell density was determined with Image J software. ANOVA P value of <0.01. E, F RKO and ARID1AOE clones were treated with or without PHA and Ferr-1. ROS intensity was analyzed with fluorescence. The fluorescence intensity was calculated with Image J software. Scale bars, 100 μm. G RKO and ARID1AOE clones were treated with or without PHA and Ferr-1. The cell viability was determined by Alamar Blue assay. Error bars represent s.d. from three independent experiments. ANOVA P value of <0.01.

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