Fig. 6: GPX4 is a target gene for transcription repression by ARID1A. | Cell Death & Differentiation

Fig. 6: GPX4 is a target gene for transcription repression by ARID1A.

From: ARID1A loss enhances sensitivity to c-MET inhibition by dual targeting of GPX4 and iron homeostasis, inducing ferroptosis

Fig. 6

A Downregulation of c-MET, SLC7A11, and GPX4 level in ARID1A-KO HCT116 cells. B Upregulation of c-MET, SLC7A11, and GPX4 level in ARID1A-overexpressing (ARID1AOE) RKO clones. C Silencing ARID1A expression decreases c-MET, SLC7A11, and GPX4 levels in HCT116 cells. D Upregulation of c-MET, SLC7A11, and GPX4 level in RKO cells by ARID1A overexpression plasmid transfection. E RT-qPCR analysis of GPX4 mRNA level in HCT116 and ARID1A-KO clones. ANOVA P < 0.01. F Four primers (Primer #1, Primer #2, Primer #3, Primer #4) were designed to cover GPX4 promoter from −1 bp to −1000 bp. G Chip of GPX4 promoter in HCT116 cells using anti-ARID1A antibody. H A representative image showing results of PCR performed on DNA samples precipitated with anti-ARID1A. I ChIP of GPX4 promoter in HCT116 cells using anti-ARID1A antibody. IgG was used as a normalization control. ANOVA P < 0.05. J ChIP of GPX4 promoter in HCT116 and ARID1A-KO cells using anti-Pol II antibody. IgG in each cell line was used as a normalization control. ANOVA P < 0.01.

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