Fig. 2: Upregulated expression of Eftud2 in SHH-subgroup of mouse and human medulloblastoma. | Cell Death & Differentiation

Fig. 2: Upregulated expression of Eftud2 in SHH-subgroup of mouse and human medulloblastoma.

From: Alternative spliceosomal protein Eftud2 mediated Kif3a exon skipping promotes SHH-subgroup medulloblastoma progression

Fig. 2

a Schematic of the RNA-seq experiment. b Volcano plots of differential gene expression in SmoM2Atoh1 mouse tumors. c KEGG enrichment analysis of differential genes in tumor of SmoM2Atoh1 mice. Western blot validation d and quantification e of crucial Spliceosome proteins and Hedgehog signaling pathway factors in cerebellum of SmoM2fl/+ mice and tumor of SmoM2Atoh1 mice (n = 6 mice, 3 males, 3 females, unpaired t-test). Immunohistochemical staining f and quantification g showed a significant upregulation of EFTUD2 expression levels in human medulloblastoma tissue microarrays in medulloblastoma patients (normal, n = 3, medulloblastoma patients, n = 18, unpaired t-test). Molecular Layer (ML), the Purkinje Layer (PL), and the Internal Granule Layer (IGL). Scale bars = 50 μm. The data are presented as the mean ± SD (bar plots). *p < 0.05, **p < 0.01, ****p < 0.0001.

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