Fig. 6: Exons 10–11 skipping in Kif3a enhances the transcriptional activity of Gli2. | Cell Death & Differentiation

Fig. 6: Exons 10–11 skipping in Kif3a enhances the transcriptional activity of Gli2.

From: Alternative spliceosomal protein Eftud2 mediated Kif3a exon skipping promotes SHH-subgroup medulloblastoma progression

Fig. 6

Western blot validation a and quantification b–d of GLI1, GLI2 and KIF3A in pGC-FU control, Scramble siRNA control, pGC-FU + Scramble siRNA control, pGC-FU + siKIF3A, siKIF3A + Kif3aR and siKIF3A + Kif3aΔE10-11 treated Daoy cells (n = 6, one-way ANOVA and Bonferroni’s multiple comparisons test). Schematic of luciferase assay e and quantification f–i of transcriptional activity of four different Gli2 downstream effectors (8GBS, Bcl2 promoter, Bcl2 enhancer, and Foxm1, n = 6, one-way ANOVA and Bonferroni’s multiple comparisons test). The data are presented as the mean ± SD (bar plots). **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. no significant.

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