Fig. 7: Exons 10–11 skipping in Kif3a promotes the proliferation of Daoy cells. | Cell Death & Differentiation

Fig. 7: Exons 10–11 skipping in Kif3a promotes the proliferation of Daoy cells.

From: Alternative spliceosomal protein Eftud2 mediated Kif3a exon skipping promotes SHH-subgroup medulloblastoma progression

Fig. 7

The effect of Kif3aR and Kif3aΔE10-11 on cellular viability was detected by CCK-8 assays a and colony formation assays b, c respectively (n = 9, two-way ANOVA and Šídák’s multiple comparisons test for CCK-8 assays, one-way ANOVA and Bonferroni’s multiple comparisons test for colony formation assays). d Immunofluorescent co-staining with anti-KIF3A and anti-KI67 in pGC-FU control, pGC-FU+siKIF3A, siKIF3A+Kif3aR and siKIF3A+Kif3aΔE10-11 treated Daoy cells. Scale bars = 50 μm and 100 μm, respectively. e EdU incorporation assays were used to identify the cells in S phase in pGC-FU control, pGC-FU+siKIF3A, siKIF3A+Kif3aR and siKIF3A+Kif3aΔE10-11 treated Daoy cells. Scale bars = 50 μm and 100 μm, respectively. Quantification of KI67+ cells f and EdU+ cells g in pGC-FU control, pGC-FU+siKIF3A, siKIF3A+Kif3aR and siKIF3A+Kif3aΔE10-11 treated Daoy cells (one-way ANOVA and Bonferroni’s multiple comparisons test). The data are presented as the mean ± SD (bar plots). *p < 0.05, ***p < 0.001, ****p < 0.0001.

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