Fig. 3: BH3-mimetics can suppress cell death induced by GPX4 inhibition.
From: Interplay of ferroptotic and apoptotic cell death and its modulation by BH3-mimetics
A, B Brightfield images with PI fluorescence overlays show cells treated for 24 h with either WEHI-539 (10 µM), RSL3 (100 nM), or both combined. C–F, H Cells were treated for 24 h with the indicated concentrations of GPX4 inhibitors (RSL3, ML-162, ML-210) in combination with BCL-XL inhibitors (WEHI-539, A-1331852), or the MCL-1 inhibitor S63845, or the BCL-2 inhibitor ABT-199. Cell death was determined by PI uptake. Antagonism was determined from the coefficient of drug interaction CDI by the Webb’s fractional product. Data represent the mean of two or three technical replicates, panels show one representative of at least two independent experiments. G Quantification of cell death, calculated as percentage of PI-positive cells. Data are the mean ± SEM of technical triplicates. One representative experiment of four independent experiments is shown.
