Fig. 2: Ferroptosis induces the production and release of inflammatory oxylipins.

A Primary mouse lung fibroblasts (PMLFs) were treated with RSL3 [1 µM] ± Ferrostatin-1 [1 µM] for 6 h. qPCR of the indicated transcripts was performed. B Parental Pfa1 or Pfa1 MEFs with stable mouse FSP1 overexpression [9] (mFSP1 OE) were treated ±4OHT [1 µM] for 36 h, and qPCR of the indicated transcripts was performed. C Pfa1 MEFs were treated - 4OHT (ctrl) or +4OHT [1 µM] ± Fer-1 [1 µM] for 72 h, supernatants and cell-free medium (n = 5 per condition) were collected, and concentrations of the indicated prostaglandins were quantified by mass spectrometry using standards as compared to media. D–H Supernatants from (C) were quantified for the indicated HETE, HODE, HETre, HDoHE, DiHOME oxylipins. Data information: A, B Graphs show data of means ± SEM of 3 independent biological replicates, and C–G 5 three independent biological replicates for lipidomics measurement. One- or two-way ANOVA was used to calculate p-values. ns: not significant; *: p < 0.05; **: p < 0.01; ***: p < 0.001; ****: p < 0.0001. Source data are available online for this figure.