Fig. 4: Cytosolic mtDNA release mediated fatty acid synthesis-related EC activation in sepsis. | Cell Death & Differentiation

Fig. 4: Cytosolic mtDNA release mediated fatty acid synthesis-related EC activation in sepsis.

From: Fatty acid synthesis promotes mtDNA release via ETS1-mediated oligomerization of VDAC1 facilitating endothelial dysfunction in sepsis-induced lung injury

Fig. 4

A KEGG analysis of genes regulated by both LPS stimulation and C75 treatment. B The levels of mtDNA in the plasma of septic patients (n = 18) were measured by RT-qPCR. C–F The correlation analysis of mtDNA with APACHE II score, SOFA score, sVCAM-1, and sE-selectin. G HUVEC were pretreated with C75 for 30 min before LPS stimuli for 4 h. Cytosolic mtDNA/nDNA was detected by RT-qPCR (n = 5). H, I HUVEC were pretreated with C75 before LPS stimuli. Confocal laser microscope assay was used to assess the levels of mtDNA (anti-dsDNA, green). Mitochondria were stained in red with Mito-Tracker and nuclei were stained in blue with DAPI. Quantitative results from 8 cells for each condition were reported. J The levels of mtDNA in the plasma of septic mice (n = 5) were measured by RT-qPCR. All data were expressed as the mean ± SD. Simple linear regression and Spearman correlation coefficients were used for (C–F). Unpaired t-test was used for the comparison between two groups. Comparison among three or more groups was analyzed by one-way ANOVA. ns no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

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