Fig. 5: Fatty acid synthesis promoted mtDNA-related activation of cGAS-STING signaling and pyroptosis in ECs in response to LPS. | Cell Death & Differentiation

Fig. 5: Fatty acid synthesis promoted mtDNA-related activation of cGAS-STING signaling and pyroptosis in ECs in response to LPS.

From: Fatty acid synthesis promotes mtDNA release via ETS1-mediated oligomerization of VDAC1 facilitating endothelial dysfunction in sepsis-induced lung injury

Fig. 5

A Heatmap of DEGs enriched in cytosolic DNA-sensing pathway. B–E HUVEC were pretreated with C75 before LPS stimuli. Protein levels of cGAS, p-STING, and p-p65 were determined by western blot (n = 3). F, G Representative immunoblots of cGAS, p-STING, and p-p65 in the lung tissues of septic mice pretreated with or without C75 (n = 3). H, I Confocal images of p65 nuclear translocation in LPS-challenged HUVEC with or without C75 pretreatment. Quantitative results from 8 cells per group are reported. All data were expressed as the mean ± SD. Comparison among three or more groups was analyzed by one-way ANOVA. ns no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

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