Fig. 8: ETS1 promoted VDAC1 oligomerization to facilitate mtDNA release and EC activation via limiting VDAC1 ubiquitination. | Cell Death & Differentiation

Fig. 8: ETS1 promoted VDAC1 oligomerization to facilitate mtDNA release and EC activation via limiting VDAC1 ubiquitination.

From: Fatty acid synthesis promotes mtDNA release via ETS1-mediated oligomerization of VDAC1 facilitating endothelial dysfunction in sepsis-induced lung injury

Fig. 8

A CoIP-MS image of VDAC1. B Molecular docking showing the interaction between ETS1 and VDAC1. C Immunoblot showing the binding of ETS1 with VDAC1 by GST pull-down (n = 3). D, E HUVEC were transfected with siRNA specific to ETS1 before LPS stimuli. The levels of VDAC1 oligomerization were detected by western blot (n = 3). F KEGG analysis of proteins binding to ETS1. G, H HUVEC were transfected with siRNA specific to ETS1 or pretreated with C75 before LPS stimuli. The ubiquitination levels of VDAC1 were detected by western blot (n = 3). All data were expressed as the mean ± SD. Comparison among three or more groups was analyzed by one-way ANOVA. ns no significance, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.

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