Fig. 3: GNE and BMS combination treatment induces the senescence-associated secretory phenotype in KRASG12V/MYC cancer cells.
From: Synergistic targeting of cancer cells through simultaneous inhibition of key metabolic enzymes
A KRASG12V/MYC cells were treated for 4 h with GNE and BMS or an adequate DMSO control, followed by analysis of gene expression by RNA-seq. A volcano plot is shown, n = 3. B KRASG12V/MYC cells underwent GNE and BMS combination treatment for specified time periods, followed by RNA-seq. The top 1500 transcripts exhibiting the highest 3rd order polynomial fits over time were k-means clustered (cluster 1 = upregulated over time, cluster 2 = downregulated over time). C The two different clusters were subjected to GSEA analysis using the KEGG database (KEGG disease database excluded). D KRASG12V/MYC cells were treated for 4 days with GNE/BMS or DMSO vehicle control and secreted factors were quantified by Olink®. A volcano plot is shown, n = 4. E Differentially secreted proteins (log2FC ≥ 1, ≤ -1; FDR ≤ 0.05) were subjected to GSEA using the KEGG database, the x-axis shows the normalized enrichment score (NES).
