Fig. 2: SEMA3G suppresses intracranial GBM growth.

A Intercellular communication networks of SEMA3s signaling pathways analyzed by CellChat (GSE162631). B Immunofluorescent staining of NRP2 in brain sections of tumor-bearing mice. Scale bar, 10 μm. C BCIP/NBT color development reveals AP-Ctrl or AP-SEMA3G binding in brain sections of tumor-bearing mice. Scale bar, 5 μm. D Schematic diagram of GBM model establishment and monitor using Sema3G^f/f and Cdh5-cre; Sema3G^f/f mice. In vivo bioluminescent image (E) and quantification (F) of tumor growth on days 7 and 21 post cell implant. Data are shown as mean ± s.e.m. n = 6, ^***P < 0.001. Data were analyzed by two-way ANOVA. Representative images (G) and the tumor-to-brain area ratio (H) based on the H&E staining mouse brains collected 21 days after transplantation of GL261 cells. Scale bar, 1 mm. Data are shown as mean ± s.e.m. n = 6, ^***P < 0.001, as determined by two-tailed unpaired t test. I Kaplan-Meier survival curves of the tumor bearing Sema3G^f/f mice and Cdh5-cre; Sema3G^f/f mice. The P values were calculated by the log-rank test. n = 6. J Schematic diagram of overexpressing SEMA3G in nude mice and the construction of a GBM model using GSC07-Luc cells. In vivo bioluminescent image (K) and quantification (L) of tumor growth in GBM bearing mice on days 7 and 21 post cell implant. Data are shown as mean ± s.e.m. n = 10, ^*P < 0.05, ^**P < 0.01. Data were analyzed by two-tailed unpaired t test. M H&E staining of mouse brains collected 21 days post GSC07 cell transplantation. Scale bar, 1 mm. n = 10. N The tumor-to-brain area ratio based on the H&E staining (M) was calculated using ImageJ software. Data are shown as mean ± s.e.m. n = 10, ^***P < 0.001, as determined by two-tailed paired t test. O Kaplan-Meier survival curves of the tumor bearing nude mice. n = 10. The P values were calculated by the log-rank test. n = 10.