Fig. 5: Caspase-8 expression and phosphorylation on Y380 ensures energy metabolism and mitochondrial activity. | Cell Death & Differentiation

Fig. 5: Caspase-8 expression and phosphorylation on Y380 ensures energy metabolism and mitochondrial activity.

From: Caspase-8 expression and its Src dependent phosphorylation on Tyrosine 380 triggers NRF2 signaling activation in glioblastoma

Fig. 5

A Representative profile of Seahorse assay showing the oxygen consumption rate (OCR) of U87 and U251 shCTR, shC8#2, shC8#2 + C8WT and shC8#2 + C8Y380F and B relative histograms of basal respiration, ATP-linked respiration, maximal respiration and spare respiratory capacity. C Cytofluorimetric analyses of active mitochondria upon MitoTracker Deep Red staining and relative quantification in U87 and U251 shCTR, shC8#2, shC8#2 + C8WT and shC8#2 + C8Y380F. D Immunoblotting of ATPB, VDAC, TOM20 and relative densitometric analyses in U87shCTR, U87shC8#2, U87shC8#2 + C8WT and U87shC8#2 + C8Y380F cells. GAPDH was used as loading control. Results represent the mean of at least three independent experiments ( ± SEM). Statistical analyses: One-way ANOVA test (B) and paired t test (C, D) (*P < 0.05; ***P < 0.001; ****P < 0.0001).

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