Fig. 6: Reconstitution of NRF2 expression rescues the mitochondrial functionality of shC8 cells. | Cell Death & Differentiation

Fig. 6: Reconstitution of NRF2 expression rescues the mitochondrial functionality of shC8 cells.

From: Caspase-8 expression and its Src dependent phosphorylation on Tyrosine 380 triggers NRF2 signaling activation in glioblastoma

Fig. 6

A Immunofluorescence of U87-MG and U251 shC8#2 and of shC8#2 + NRF2 cells; NRF2 (green) and DNA (Hoechst, blue); B Immunoblotting of NRF2 and target proteins p62, HO1 and NQO1 and relative densitometric analyses in the same cells in A. Vinculin was used as loading control. C Histograms of basal respiration, ATP-linked respiration, maximal respiration and spare respiratory capacity by Seahorse assay showing the oxygen consumption rate (OCR) in U87-MG and U251 shC8#2 and of shC8#2 + NRF2 cells. D Cytofluorimetric analyses of active mitochondria upon MitoTracker Deep Red staining in U87-MG and U251 shC8#2 and of shC8#2 + NRF2 cells. E Immunofluorescence of U87-MG and U251 shC8#2 and of shC8#2 + NRF2; TOM20 (red) and DNA (Hoechst, blue). F Working model. Results represent the mean of at least three independent experiments ( ± SEM). Statistical analyses: paired t test (B, D) and One-way ANOVA test (C) (*P < 0.05; ***P < 0.001; ****P < 0.0001).

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