Fig. 1: TMAO exacerbates renal fibrosis in UUO mice. | Cell Death & Differentiation

Fig. 1: TMAO exacerbates renal fibrosis in UUO mice.

From: Intestinal metabolite TMAO promotes CKD progression by stimulating macrophage M2 polarization through histone H4 lysine 12 lactylation

Fig. 1

In view of the high consistency among the same mouse species, 30 mice were divided into 6 groups, each consisting of 5 mice. The Normal group received no treatment, the Sham group underwent a laparotomy procedure, the Sham+TMAO group underwent the laparotomy procedure and received drinking water containing 0.3% TMAO, the UUO group underwent left kidney ureteral ligation, the UUO + TMAO group underwent left kidney ureteral ligation and received drinking water containing 0.3% TMAO, and the UUO + IMC group underwent left kidney ureteral ligation and received drinking water containing the TMAO inhibitor IMC at 0.06%, the intervention continued for 14 days. A The level of TMA, TMAO, Betaine, Creatinine, Carnitine, Choline in urine of different groups of mice. B Masson stain of the left kidney of different groups of mice. C α-SMA, collagen I, fibronectin, LDHA, PDH mRNA expression in the left kidney of different groups of mice. D Western blotting showing the α-SMA, collagen I, fibronectin, LDHA, PDH levels of the left kidney of different groups of mice. E IL-12, iNOS, IL-6, IL-1β, and TNF-α mRNA expression in the left kidney of different groups of mice. F Arg-1, Fizz1, YM-1, TGFβ1, MR, IL-10 mRNA expression in the left kidney of different groups of mice. G Elisa showing the Cystatin C, Creatinine, BUN, LD, Acetyl-CoA level in the serum, and the urine protein.

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