Fig. 1: High expression of METTL14 was associated with adverse outcomes in JMML patients, while its knockout significantly inhibited disease progression.

Control, Kras, and Kras;M14-/- mice were sacrificed on day 1 of week seven for analysis of different hematopoietic tissues. A METTL14 expression in normal donors and JMML patients in GSE71935. B Kaplan-Meier plot. Survival curves for high (red) and low (blue) expression groups dichotomized at the optimal cutpoint are plotted and analysis of overall survival rate in the mutated cases of dataset GSE71935. C, D METTL14 mRNA expression and protein level in Kras, and Kras;M14-/- mice. E Quantification of total RNA m6A levels in Lin-c-Kit+ cells and bone marrow cells. F Kaplan-Meier survival curves of different groups of mice were plotted after birth. P values were determined by the Log-rank test. G Quantification of body weight. H–J CBC analysis results. Numbers of RBC(red blood cell),WBC (white blood cell), PLT(blood platelet) are shown. K myeloid cell compartment in BM, SP and PB. The results are presented as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.