Fig. 2: Knockout of METTL14 rescues KrasG12D/+-mediated HSPCs depletion. | Cell Death & Differentiation

Fig. 2: Knockout of METTL14 rescues KrasG12D/+-mediated HSPCs depletion.

From: The m6A methyltransferase METTL14 promotes oncogenic Kras induced juvenile myelomonocytic leukemia through dysregulating autophagy

Fig. 2

Control, Kras, and Kras;M14-/- mice were sacrificed on day 1 of week seven for analysis of Lin-c-Kit+ C, HSCs D, MPPs E, MPs F and CLPs in hind limb BM (H.L.) and SP. A Representative example of staining and gating for HSCs, MPPs, Lin-c-Kit+cells, LSKs and MPs. B Representative example of staining and gating for CLPs. C–G The absolute Lin-c-Kit+cells, HSCs, MPPs, MPs and CLPs numbers in BM (H.L.) and SP were calculated based on BM or SP cell numbers and frequencies. Because BM (H.L.) represents 25% of whole body bone marrow, the total number of stem/progenitor cells per animal was calculated as the sum of stem/progenitor cell number in SP and four fold of stem/progenitor cell number in BM (H.L.). The results are presented as means ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001.

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