Table 1 Assessment of inappropriate cell survival by counting cell number in the anterior pharynx.

From: Fluorescent protein tagging of C. elegans core apoptosis pathway components reveals mitochondrial localization of CED-9 Bcl-2, CED-4 Apaf1 and CED-3 Caspase in non-apoptotic and apoptotic cells

Genotype

Average number of extra cells per pharynx

Number of animals scored

p value

N2

0

15

NA

ced-3(dx228)

0.21

14

<0.05

ced-4(dx226)

0.07

14

>0.05

ced-9(dx236)

0

12

>>0.05

  1. p values were calculated using a one-tailed z-test and indicate the likelihood that the observed number of surviving cells is not greater than observed in N2 [56]. These calculations are based on the observation that Ced animals have an average of 11.4 extra cells in the anterior pharynx [8]. Sample sizes were chosen to allow detection of a significant increase in the number of surviving cells at a frequency of ≥2%. Each CRISPR-edited strain was outcrossed once to N2 prior to scoring.
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