Fig. 6: CCDC719-13 interacts with SLC7A11 and modulates ferroptosis pathway in prostate cancer cells. | Cell Death & Differentiation

Fig. 6: CCDC719-13 interacts with SLC7A11 and modulates ferroptosis pathway in prostate cancer cells.

From: Induction of ferroptosis in prostate cancer by CCDC719-13 via TRIM21-mediated ubiquitination of SLC7A11

Fig. 6

A Immunoprecipitation (IP) analysis using anti-Flag antibody demonstrates the presence of Flag-CCDC71913 in PC3 cells, indicating successful overexpression and capture. B Co-immunoprecipitation (co-IP) showing the interaction between Flag-CCDC719-13 and SLC7A11. C Mass spectrometry analysis confirms the interaction between CCDC719-13 and SLC7A11 with a significant peptide spectrum match. D Western blot analysis of IP samples from PC3 and DU145 cells confirming the interaction between CCDC719-13 and SLC7A11. E Immunofluorescence microscopy of PC3 and DU145 cells treated with MG132, showing colocalization of CCDC719-13 (red) and SLC7A11 (green), with nuclei stained by DAPI (blue). F Co-IP in HEK293 cells co-transfected with CCDC719-13 and Myc-SLC7A11 confirms their interaction. G Schematic representation of SLC7A11 truncation mutants (SLC7A11-N and SLC7A11-C) used for mapping the interaction domain with CCDC719-13. H GST pull-down assay using GST-tagged SLC7A11 truncation mutants and Flag-CCDC719-13. I Western blot analysis of PC3 and DU145 cells showing the effect of CCDC719-13 overexpression on the expression levels of SLC7A11, GPX4, and MDA. J Western blot analysis of PC3 and DU145 cells co-transfected with CCDC719-13 and SLC7A11, showing the effect on ferroptosis markers.

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