Fig. 9: CCDC719-13/TRIM21 catalyze K48-linked polyubiquitination of SLC7A11 at K12. | Cell Death & Differentiation

Fig. 9: CCDC719-13/TRIM21 catalyze K48-linked polyubiquitination of SLC7A11 at K12.

From: Induction of ferroptosis in prostate cancer by CCDC719-13 via TRIM21-mediated ubiquitination of SLC7A11

Fig. 9

A Analysis of ubiquitin linkage types on SLC7A11. PC3 cells were co-transfected with Myc-SLC7A11, Flag-CCDC719-13, and HA-tagged ubiquitin (WT, K6, K11, K27). Cells were treated with MG132 (10 µM) to inhibit proteasomal degradation. Myc-SLC7A11 was immunoprecipitated, and the presence of HA-tagged ubiquitin was detected by Western blotting. B Ubiquitin linkage specificity of SLC7A11 in the presence of CCDC719-13. PC3 cells were co-transfected with Myc-SLC7A11, Flag-CCDC719-13, and HA-tagged ubiquitin (K29, K33, K48, K63). C Ubiquitination analysis of SLC7A11 in PC3 and DU145 cells transfected with Flag-SLC7A11, HA-tagged ubiquitin (WT, K48R, K63R), and Flag-CCDC719-13. D Analysis of SLC7A11 ubiquitination in the presence of wild-type and K12R mutant forms of Myc-SLC7A11. Co-immunoprecipitation (Co-IP) analysis of SLC7A11, CCDC719-13, and TRIM21 in PC3 (E) and DU145 (F) cells. Immunoprecipitation was performed using anti-Flag, anti-HA, or control IgG antibodies.

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