Fig. 3: UGDH O-GlcNAcylation impacts the tumor immune cell landscape. | Cell Death & Differentiation

Fig. 3: UGDH O-GlcNAcylation impacts the tumor immune cell landscape.

From: O-GlcNAcylation of UGDH regulates its activity and remodels the extracellular matrix to facilitate tumor growth

Fig. 3

A A flow chart illustrates tissue processing and CyTOF analysis of lung tumors generated by subcutaneous injection. Two-dimensional T-distributed stochastic neighbor embedding (t-SNE) scatterplot of the CyTOF-TILs data derived from UGDH-WT (B) and UGDH-S350A (C) tumor showed that 8 main clusters were identified with corresponding markers. D Comparing the contributions of various clusters of immune cells from total CD45+ lymphocytes between the two groups of samples. E Representative immunohistochemistry images and quantification of CD8+ T cells in primary tumors formed by UGDH-WT and UGDH-S350A NSCLC cells. Scale bar, 100 μm. F t-SNE re-analysis of CD8+ T cells colored by relative expression of CyTOF markers, with clusters indicated. G Comparing the percentage of Granzyme B+CD8+ T cells in lymphocytes between UGDH-WT and UGDH-S350A tumors.

Back to article page