Fig. 7: UGDH O-GlcNAcylation suppresses the nuclear translocation of STAT1. | Cell Death & Differentiation

Fig. 7: UGDH O-GlcNAcylation suppresses the nuclear translocation of STAT1.

From: O-GlcNAcylation of UGDH regulates its activity and remodels the extracellular matrix to facilitate tumor growth

Fig. 7

A Nuclear and cytoplasmic expression of STAT1 and p-STAT1 in UGDH WT and UGDH S350A LLC cells with or without IFNγ treatment. B, C STAT1 nuclear localization and quantification in rWT and rS350A LLC cells with or without IFNγ treatment (n = 3). D Immunoblotting analysis of the association of HA-tagged UGDH with Flag-tagged STAT1. Immunoblotting analysis of the association of Flag-tagged KPNA2 (E) and His-tagged KPNB1 (F) with HA-tagged UGDH in HEK293T cells. G, H Immunoblotting analysis of the association of KPNA2 with UGDH WT and UGDH S350A in HEK293T cells in the presence of TMG or OSMI-4. I Immunoblotting analysis of the association of Flag-tagged KPNA2 with the endogenous STAT1 in HEK293T cells in the presence of UGDH WT or UGDH S350A. J Immunoblotting analysis of the association of Myc-tagged KPNA2 and Flag-tagged STAT1 in HEK293T cells in the presence of HA-UGDH WT or HA-UGDH S350A. K Nuclear and cytoplasmic STAT1 and p-STAT1 expressions in UGDH WT or UGDH-KO cells after IFNγ treatment. L Immunoblotting analysis of the association of endogenous STAT1 and KPNA2 in UGDH WT and KO cells with IFNγ treatment. Data are presented as means ± SEM. Statistical analyses were performed by an unpaired two-tailed Student’s t-test.

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