Fig. 6: Lig3, Lig1, and PARP1 gene silencing in NCSC-MYCN results in loss of the NCSC-like state | Cell Death & Disease

Fig. 6: Lig3, Lig1, and PARP1 gene silencing in NCSC-MYCN results in loss of the NCSC-like state

From: Alternative NHEJ pathway proteins as components of MYCN oncogenic activity in human neural crest stem cell differentiation: implications for neuroblastoma initiation

Fig. 6

For genetic knockdown, NCSC-MYCN cells were transfected with either siLig3, siLig1, or siPARP1 with siCtrl as scrambled non-targeting control. a Enzymatic depletion was confirmed by qRT-PCR. NCSC-MYCN with siLig3, siLig1 and siPARP1 had significant decrease of respective mRNA levels compared to siCtrl. b Protein expression analysis of siLig3, siLig1 and siPARP1 cells also showed significant decrease in the respective enzymes. c and d Immunofluorescence and Western blot analysis for protein expression levels of MYCN and p75 in siLig3, siLig1, and siPARP1 showed significant decrease in expression of p75 in all three subsets compared to siCtrl. No differences were observed in MYCN expression patterns

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